Tuesday, August 9, 2011

Chromatography and Flames

Chromatography Experiment!

Procedure:
1. Cut rectangular pieces of each digit out of cheque paper, keeping in mind order of digits.
2. Use forceps to transfer each piece into each Microfuge tube.
3. Pour a little ethanol (NOT TOO MUCH) into Microfuge tubes, just enough to cover digits.
4. Gently shake Microfuge tubes.
5. Draw pencil line 2 cm from width of each strip of chromatography paper and label each one (e.g marker ink, digit no. 1)
6. Use capillary tube to transfer ink from each Microfuge tube to the pencil line of each chromatography paper strip.
7. Use the marker (i.e. 1 or 2) to dot on one of the chromatography papers.
6. Fill each boiling tubes with 1ml of distilled water.
7. Dip each chromatography paper into each boiling tube so that the pencil line is a little above the water level. Use wooden peg to hold the paper in place.
8. When the solvent is about to reach the wooden peg, quickly remove the paper from the boiling tubes and mark the solvent fronts.
9. Calculate Rf values and compare.

PICTURES! (:








Flames hands-on!
Copper (II) Nitrate



Barium Nitrate




Sodium Nitrate




Lead (II) Nitrate




Potassium Nitrate



Calcium Chloride





Fascinating pictures of MS TAN conducting some experiments!



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